Production of Botulinum Toxin A

known to man, also employs a vital purpose in medical drug therapy. Through the growth, extraction, and purification of Botulinum Toxin A (BT-A) from either live cultures or spores of C. botulinum, patients suffering from a multitude of neuromuscular disorders are given more freedom to live independently and painlessly. Specifically, in the neuromuscular disorder Cerebral Palsy, an excess release of acetylcholine results in uncontrolled muscle contraction. BT-A inhibits the transfer of acetylcholine in the neuromuscular junction and thereby prevents muscle contraction. Methods: Commonly used methods for BT-A production begin with growing and fermenting C. botulinum spores until reaching a specified concentration. The toxin itself is removed from the fermented beer through acid precipitation and centrifugation. Multiple types of chromatography can be used for further purification of the toxin, and the concentrated substance is fully polished through crystallization. The final product is diluted in a human serum medium as a liquid or freeze dried to store in the solid state. Objective: Our purpose is to develop an efficient process for mass producing BT-A for medicinal uses. Production of Botulinum Toxin A